Resveratrol induces intracellular Ca2+ rise via T-type Ca2+ channels in a mesothelioma cell line

This study was designed to determine whether Res affects Ca2+ signaling in cancer cells. Main methods We used the REN human mesothelioma cell line, as an in vitro cancer cell model, and the non-malignant human mesothelial MeT5A cell line, as normal cell model. Cytosolic Ca2+ concentration was measured by the fluorescent indicator Fura-2. Immunofluorescence, Western blot, and siRNA technique were employed to assess the involvement of T-type Ca2+ channels. Cell viability was determined by the calcein assay. Key findings REN cells transiently exposed to 1–10μM Res showed increasing peaks of Ca2+ that were absent in Ca2+-free medium and were reduced by non-selective (Ni2+), and highly selective (NNC 55-0396) T-type Ca2+ channels antagonist, and by siRNA knockout of Cav3.2T-type Ca2+ chann...

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