Therapeutic approach to target mesothelioma cancer cells using the Wnt antagonist, secreted frizzled-related protein 4: Metabolic state of cancer cells.

In this study, we determined the effect of secreted frizzled-related protein 4 (sFRP4), a Wnt pathway inhibitor, on cancer cell proliferation and metabolism using the JU77 mesothelioma cell line. Treatment with sFRP4 (250pg/ml) resulted in a significant reduction of cell proliferation. The addition of the Wnt activator Wnt3a (250pg/ml) or sFRP4 had no significant effect on ATP production and glucose utilization in JU77 cells at both the 24 and 48hour time points examined. We also examined their effect on Akt and Glycogen synthase kinase-3 beta (GSK3β) phosphorylation, which are both important components of Wnt signalling and glucose metabolism. We found that protein phosphorylation of Akt and GSK3β varied over the 24 hour and 48 hour time points, with constitutive phosphorylation of Akt ...

from MedWorm: Mesothelioma http://www.medworm.com/index.php?rid=165813439&cid=c_409_171_f&fid=35561&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F26868304%3Fdopt%3DAbstract